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產(chǎn)品信息

產(chǎn)品性質(zhì)

品牌 Spirochrome

λabs 619 nm

λfl 636 nm

推薦STED損耗激光波長 775 nm

lifetime 2.9 ns

運輸 常溫

儲存 -20℃

能否用于固定細(xì)胞 支持任何方式固定的細(xì)胞

產(chǎn)品簡介

SPY620-BG is a bright, cell permeable and red emitting benzylguanine substrate for labeling SNAP-tag?* in cellular fluorescence imaging experiments.

Product contains 1 vial of SPY620-BG (35 nmol) in lyophilised form allowing to prepare 35 ul of 400x DMSO stock solution.

SPY620-BG is the benzylguanine derivative of our SPY620 fluorophore. It emits light in the orange part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY620-BG can be imaged with e.g. using 610/20 nm and 650/40 nm excitation and emission filters respectively. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue.

Benzylguanine (BG) is the substrate of the self labeling tag SNAP-tag?*. Upon reaction with a BG derivative, SNAP-tag?* forms a covalent bond with the substrate and releases guanine. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as SNAP-tag?* fusion.

*SNAP-tag? is a registered trademark of New England Biolabs, Inc.

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